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Kim JJ, Chan PPY, Vlassakis J, Geldert A, Herr AE. Microparticle Delivery of Protein Markers for Single-Cell Western Blotting from Microwells. Small. 2018 Nov;14(48):e1802865. doi: 10.1002/smll.201802865. Epub 2018 Oct 17.

Kim JJ, Chan PPY, Vlassakis J, Geldert A, Herr AE. Microparticle Delivery of Protein Markers for Single-Cell Western Blotting from Microwells. Small. 2018 Nov;14(48):e1802865. doi: 10.1002/smll.201802865. Epub 2018 Oct 17.

为了在单细胞蛋白质印迹中结合蛋白质标记物，使用微孔分离单个细胞和蛋白质标记物包被的微粒。

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Wang Z, Zhang X. Single Cell Proteomics for Molecular Targets in Lung Cancer: High-Dimensional Data Acquisition and Analysis. Adv Exp Med Biol. 2018;1068:73-87. doi: 10.1007/978-981-13-0502-3_7. PMID: 29943297.

Wang Z, Zhang X. Single Cell Proteomics for Molecular Targets in Lung Cancer: High-Dimensional Data Acquisition and Analysis. Adv Exp Med Biol. 2018;1068:73-87. doi: 10.1007/978-981-13-0502-3_7. PMID: 29943297.

与传统的蛋白质组学方法相比，单细胞蛋白质组学方法可以更好地实现这一点。

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Kaelberer MM, Buchanan KL, Klein ME, Barth BB, Montoya MM, Shen X, Bohórquez DV. A gut-brain neural circuit for nutrient sensory transduction. Science. 2018 Sep 21;361(6408):eaat5236. doi: 10.1126/science.aat5236. PMID: 30237325; PMCID: PMC6417812.

Kaelberer MM, Buchanan KL, Klein ME, Barth BB, Montoya MM, Shen X, Bohórquez DV. A gut-brain neural circuit for nutrient sensory transduction. Science. 2018 Sep 21;361(6408):eaat5236. doi: 10.1126/science.aat5236. PMID: 30237325; PMCID: PMC6417812.

我们对大脑神经回路的理解正随着具有高度地形和时间精度的分子工具的出现而向前推进。我们对这些工具进行了调整，以便在肠道中使用。单细胞定量 PCR 和 Milo 单细胞 Westernblot 可以评估突触蛋白。

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Sinkala E, Sollier-Christen E, Renier C, Rosàs-Canyelles E, Che J, Heirich K, Duncombe TA, Vlassakis J, Yamauchi KA, Huang H, Jeffrey SS, Herr AE. Profiling protein expression in circulating tumour cells using microfluidic western blotting.

Sinkala E, Sollier-Christen E, Renier C, Rosàs-Canyelles E, Che J, Heirich K, Duncombe TA, Vlassakis J, Yamauchi KA, Huang H, Jeffrey SS, Herr AE. Profiling protein expression in circulating tumour cells using microfluidic western blotting.

Single cell WB 具有的痕量检测特点，可在 一次普通的抽血中（2-4 ml 血液），检测到单个循环肿瘤细胞（CTC）多种蛋白标志物，为液体活检提供更精准的诊断标志物。

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Duncombe TA, Kang CC, Maity S, Ward TM, Pegram MD, Murthy N, Herr AE. Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting. Adv Mater. 2016 Jan 13;28(2):327-334. doi: 10.1002/adma.201503939.

Duncombe TA, Kang CC, Maity S, Ward TM, Pegram MD, Murthy N, Herr AE. Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting. Adv Mater. 2016 Jan 13;28(2):327-334. doi: 10.1002/adma.201503939.

大多数癌症疗法靶向蛋白质和肿瘤异质性驱动药物反应和耐药性。单细胞分辨率蛋白质组学工具可以提供新的见解。

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Kang CC, Yamauchi KA, Vlassakis J, Sinkala E, Duncombe TA, Herr AE. Single cell-resolution western blotting. Nat Protoc. 2016 Aug;11(8):1508-30. doi: 10.1038/nprot.2016.089. Epub 2016 Jul 28. PMID: 27466711; PMCID: PMC5511750.

Kang CC, Yamauchi KA, Vlassakis J, Sinkala E, Duncombe TA, Herr AE. Single cell-resolution western blotting. Nat Protoc. 2016 Aug;11(8):1508-30. doi: 10.1038/nprot.2016.089. Epub 2016 Jul 28. PMID: 27466711; PMCID: PMC5511750.

描述了如何对单个细胞进行蛋白质免疫印迹以测量蛋白质表达水平和蛋白质状态的细胞间变异。与传 统的蛋白质印迹一样，单细胞蛋白质免疫印迹（scWB）对于缺乏选择性抗体（例如，亚型）的蛋白质靶标 以及来自完整细胞的背景信号混淆的情况特别有用。

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Quadri SM. Single-cell western blotting. Methods Mol Biol. 2015;1312:455-64. doi: 10.1007/978-1-4939-2694-7_46. PMID: 26044027.

Quadri SM. Single-cell western blotting. Methods Mol Biol. 2015;1312:455-64. doi: 10.1007/978-1-4939-2694-7_46. PMID: 26044027.

为了检测这种细胞间变异，进行了一种称为“单细胞蛋白质印迹”的高度先进的技术。

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Hughes AJ, Spelke DP, Xu Z, Kang CC, Schaffer DV, Herr AE. Single-cell western blotting. Nat Methods. 2014 Jul;11(7):749-55. doi: 10.1038/nmeth.2992. Epub 2014 Jun 1. PMID: 24880876; PMCID: PMC4077215.

Hughes AJ, Spelke DP, Xu Z, Kang CC, Schaffer DV, Herr AE. Single-cell western blotting. Nat Methods. 2014 Jul;11(7):749-55. doi: 10.1038/nmeth.2992. Epub 2014 Jun 1. PMID: 24880876; PMCID: PMC4077215.

支持 30μm 厚光敏聚丙烯酰胺凝胶的显微镜载玻片可实现蛋白质印迹：将单个细胞沉降到微孔中，原 位裂解，凝胶电泳，光诱导印迹以固定蛋白质和抗体探测。

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